More than 40 renowned authorities carefully introduce and update chapters on the theory, fundamentals, techniques, and instrumentation of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC), highlighting the latest procedures and applications of TLC to 19 important compound classes. It is one of the only books available with coverage of TLC applications by compound type. Facilitating industrial adaptation through easy reference, the second edition supports practical research strategies with extensive tables of data, offers numerous figures that illustrate techniques and chromatograms, and includes a glossary as well as a directory of equipment suppliers.

There is a dramatic rise of novel drug use due to the increased popularity of so-called designer drugs. These synthetic drugs can be illegal in some countries, but legal in others and novel compounds unknown to drug chemistry emerge monthly. This thoughtfully constructed edited reference presents the main chromatographic methodologies and strategies used to discover and analyze novel designer drugs contained in diverse biological materials. The methods are based on molecular characteristics of the drugs belonging to each individual class of compounds, so it will be clear how the current methods are adaptable to future new drugs that appear in the market.

Thin layer chromatography (TLC) is increasingly used in the fields of plant chemistry, biochemistry, and molecular biology. Advantages such as speed, versatility, and low cost make it one of the leading techniques used for locating and analyzing bioactive components in plants. Thin Layer Chromatography in Phytochemistry is the first source devoted to supplying state-of-the-art information on TLC as it applies to the separation, identification, quantification, and isolation of medicinal plant components. Renowned scientists working with laboratories around the world demonstrate the applicability of TLC to a remarkable diversity of fields including plant genetics, drug discovery, nutraceuticals, and toxicology. Elucidates the role of plant materials in the pharmaceutical industry... Part I provides a practical review of techniques, relevant materials, and the particular demands for using TLC in phytochemical applications. The text explains how to determine the biological activity of metabolites and assess the effectiveness of herbal medicines and nutritional supplements. Part II concentrates on TLC methods used to analyze specific plant-based metabolite classes such as carbohydrates, proteins, alkaloids, flavonoids, terpenes, etc. Organized by compound type, each chapter discusses key topics such as sample preparation, plate development, zone detection, densitometry, and biodetection. Demonstrates practical methods that can be applied to a wide range of disciplines... From identification to commercial scale production and quality control, Thin Layer Chromatography in Phytochemistry is an essential bench-top companion and reference on using TLC for the study of plant-based bioactive compounds.

Chemometrics uses advanced mathematical and statistical algorithms to provide maximum chemical information by analyzing chemical data, and obtain knowledge of chemical systems. Chemometrics significantly extends the possibilities of chromatography and with the technological advances of the personal computer and continuous development of open-source software, many laboratories are interested in incorporating chemometrics into their chromatographic methods. This book is an up-to-date reference that presents the most important information about each area of chemometrics used in chromatography, demonstrating its effective use when applied to a chromatographic separation.

HPLC is the principal separation technique for identification of the pesticides in environmental samples and for quantitative analysis of analytes. At each stage of the HPLC procedure, the chromatographer should possess both the practical and theoretical skills required to perform HPLC experiments correctly and to obtain reliable, repeatable, and reproducible results. Developed to serve as a detailed practical guide, High Performance Liquid Chromatography in Pesticide Residue Analysis is a comprehensive source of information and training on state-of-the-art pesticide residue methods performed with the aid of HPLC. The book presents the pros and cons of HPLC as a flexible and versatile separation and analysis tool with multiple purposes and advantages in investigations of pesticides for food and plant drugs standardization, promotion of health, protection of new herbal medicines, and more.

Preparative Layer Chromatography explains how this method is used for separating large quantities of mixtures containing a wide variety of important compounds. It offers a broad review of preparative layer chromatography (PLC) applications and adaptable working procedures for microseparations involving organic, inorganic, and organometallic compounds. The book contains theoretical background, chemical principles, and relevance of preparative layer chromatography (PLC) to a wide range of applications, particularly in the study of pharmaceuticals and biochemistry. Written by many of the best known and most knowledgeable specialists in the field, the chapters describe all the necessary techniques, current procedures, and superior strategies for selecting the most suitable eluents and designing application-specific PLC systems based on the data being sought. They provide comprehensive instructions, surrounding issues, and suggestions for optimizing optional working techniques within the framework of PLC. The book also provides a complete coverage of bulk sorbents and precoated chromatographic plates available on the international market. A comprehensive, yet accessible source of information, Preparative Layer Chromatography is a relevant and practical text for experienced as well as novice researchers and practitioners involved in analytical, environmental, geochemical, biological, medicinal, and pharmaceutical analysis.

Thin layer chromatography (TLC) is well suited for performing enantioseparations for research as well as larger-scale applications. A fast, inexpensive, and versatile separation technique, there are many practical considerations that contribute to its effectiveness. Thin Layer Chromatography in Chiral Separations and Analysis is the first book to focus solely on the theory, capabilities, and applications of TLC for direct and indirect enantioseparations. The first part of the book examines the fundamental principles of chirality and TLC. It describes the necessary materials, laboratory equipment, procedures, and strategies for the separation, quantification, isolation, and analysis of chiral compounds. The second part evaluates the real-world enantioseparations and densitometric analyses. Emphasizing pharmaceutical applications, the book discusses chiral separation mechanisms and methods for analyzing the chiral purity of diastereoisomers, amino acids, beta-blockers, and NSAIDS. Topics also include commercial stationary phases and chiral modifiers of mobile phases. Thin Layer Chromatography in Chiral Separations and Analysis presents a unified perspective of theory and experimental details underlying the collective developments in the field. The book offers scientists in a variety of disciplines and levels of expertise a complete guide to understanding the current and potential applications of chiral TLC.

The CRC Handbook of Chromatography is a series of work-bench references for scientists and researchers using chromatographic systems for the analysis of organic and inorganic compounds. This handbook is an assemblage of tables where, besides data obtained by modern separation methods, older sources often difficult to access have been included to give maximum information. For use in scientific research and routine analysis where the exact determination of plant pigments, because of their light absorbing properties and defined tasks, is necessary.

CRC Handbook of Chromatography: Carbohydrates, Volume II updates the first volume, continuing coverage of literature published from 1979 to 1989. Tabulated for easy reference and thoroughly documented, it presents the comprehensive data for all chromatographic techniques applicable to carbohydrates. It features glycoproteins, proteoglycans, and glycolipids, as well as mono-, oligo-, and polysaccharides. This important text emphasizes novel chromatographic methods. Highlights of this superb work include the diversity of HPLC methods applicable to carbohydrates, and the use of some new techniques, including supercritical fluid chromatography and ion chromatography in carbohydrate analysis. Readers discover the latest detection methods, degradative processes, and derivatization techniques. Detailed chapters cover topics such as spectroscopic techniques, electrochemistry, and gas chromatography. This easy-to-use volume provides an excellent working manual and reference book for researchers in the fields of carbohydrate chemistry and biochemistry. CRC Handbook of Chromatography, Carbohydrates: Volume II is an absolute must for all analysts working for industries concerned with carbohydrates.

CRC Handbook of Chromatography: Carbohydrates, Volume II updates the first volume, continuing coverage of literature published from 1979 to 1989. Tabulated for easy reference and thoroughly documented, it presents the comprehensive data for all chromatographic techniques applicable to carbohydrates. It features glycoproteins, proteoglycans, and glycolipids, as well as mono-, oligo-, and polysaccharides. This important text emphasizes novel chromatographic methods. Highlights of this superb work include the diversity of HPLC methods applicable to carbohydrates, and the use of some new techniques, including supercritical fluid chromatography and ion chromatography in carbohydrate analysis. Readers discover the latest detection methods, degradative processes, and derivatization techniques. Detailed chapters cover topics such as spectroscopic techniques, electrochemistry, and gas chromatography. This easy-to-use volume provides an excellent working manual and reference book for researchers in the fields of carbohydrate chemistry and biochemistry. CRC Handbook of Chromatography, Carbohydrates: Volume II is an absolute must for all analysts working for industries concerned with carbohydrates.

Planar Chromatography-Mass Spectrometry focuses on a relatively new approach to chemical analysis in general, and to separation science in particular. It is the first book to systemically cover the theoretical background, techniques, instrumentation, and practical applications of planar chromatography-mass spectrometry as a hyphenated tool of analytical chemistry. It also examines the high and as-yet unexploited potential of planar chromatography-mass spectrometry for analytical use in scientific investigations. This book overviews the combination of planar chromatography, a relatively simple and cost-effective separation step for determining complex mixtures of compounds, with mass spectrometry, an efficient, highly instrumental, and relatively expensive technique that enables rapid identification of separated chemical species. It covers electrophoretic-mass spectrometry methods and applications, which are considered planar chromatographic techniques and are increasingly being exploited in proteomic and molecular biology studies as well as for medical diagnostic purposes. It also provides a selection of applications, such as drug control and forensic and food analysis, including more difficult substances such as carbohydrates and lipids. The book advocates growth in using planar chromatography-mass spectrometry in laboratories that have appropriate equipment but have not yet employed the techniques in combination. It also describes the use of a relatively inexpensive commercial system that can be adopted by laboratories currently working without the coupled methodology. Aiming to improve power and efficiency when other analytical methods are inadequate, Planar Chromatography-Mass Spectrometry encourages separation science practitioners in academia and industry to combine the two methods for enhanced results.

Xenobiotics are chemical compounds foreign to a given biological system. In animals and humans, xenobiotics include drugs, drug metabolites, and environmental pollutants. In the environment, xenobiotics include synthetic pesticides, herbicides, and industrial pollutants. Many techniques are used in xenobiotics residue analysis; the method selected depends on the complexity of the sample, the nature of the matrix/analytes, and the analytical techniques available. This reference will help the analyst develop effective and validated analytical strategies for the analysis of hundreds of different xenobiotics on hundreds of different sample types, quickly, accurately and at acceptable cost.

Planar Chromatography-Mass Spectrometry focuses on a relatively new approach to chemical analysis in general, and to separation science in particular. It is the first book to systemically cover the theoretical background, techniques, instrumentation, and practical applications of planar chromatography-mass spectrometry as a hyphenated tool of analytical chemistry. It also examines the high and as-yet unexploited potential of planar chromatography-mass spectrometry for analytical use in scientific investigations. This book overviews the combination of planar chromatography, a relatively simple and cost-effective separation step for determining complex mixtures of compounds, with mass spectrometry, an efficient, highly instrumental, and relatively expensive technique that enables rapid identification of separated chemical species. It covers electrophoretic-mass spectrometry methods and applications, which are considered planar chromatographic techniques and are increasingly being exploited in proteomic and molecular biology studies as well as for medical diagnostic purposes. It also provides a selection of applications, such as drug control and forensic and food analysis, including more difficult substances such as carbohydrates and lipids. The book advocates growth in using planar chromatography-mass spectrometry in laboratories that have appropriate equipment but have not yet employed the techniques in combination. It also describes the use of a relatively inexpensive commercial system that can be adopted by laboratories currently working without the coupled methodology. Aiming to improve power and efficiency when other analytical methods are inadequate, Planar Chromatography-Mass Spectrometry encourages separation science practitioners in academia and industry to combine the two methods for enhanced results.

Used routinely in drug control laboratories, forensic laboratories, and as a research tool, thin layer chromatography (TLC) plays an important role in pharmaceutical drug analyses. It requires less complicated or expensive equipment than other techniques, and has the ability to be performed under field conditions. Filling the need for an up-to-date, complete reference, Thin Layer Chromatography in Drug Analysis covers the most important methods in pharmaceutical applications of TLC, namely, analysis of bulk drug material and pharmaceutical formulations, degradation studies, analysis of biological samples, optimization of the separation of drug classes, and lipophilicity estimation. The book is divided into two parts. Part I is devoted to general topics related to TLC in the context of drug analysis, including the chemical basis of TLC, sample pleparation, the optimization of layers and mobile phases, detection and quantification, analysis of ionic compounds, and separation and analysis of chiral substances. The text addresses the newest advances in TLC instrumentation, two-dimensional TLC, quantification by slit scanning densitometry and image analysis, statistical processing of data, and various detection and identification methods. It also describes the use of TLC for solving a key issue in the drug market-the presence of substandard and counterfeit pharmaceutical products. Part II provides an in-depth overview of a wide range of TLC applications for separation and analysis of particular drug groups. Each chapter contains an introduction about the structures and medicinal actions of the described substances and a literature review of their TLC analysis. A useful resource for chromatographers, pharmacists, analytical chemists, students, and R&D, clinical, and forensic laboratories, this book can be utilized as a manual, reference, and teaching source.

The CRC Handbook of Chromatography is a series of work-bench references for scientists and researchers using chromatographic systems for the analysis of organic and inorganic compounds. This handbook is an assemblage of tables where, besides data obtained by modern separation methods, older sources often difficult to access have been included to give maximum information. For use in scientific research and routine analysis where the exact determination of plant pigments, because of their light absorbing properties and defined tasks, is necessary.

Thin layer chromatography (TLC) is increasingly used in the fields of plant chemistry, biochemistry, and molecular biology. Advantages such as speed, versatility, and low cost make it one of the leading techniques used for locating and analyzing bioactive components in plants. Thin Layer Chromatography in Phytochemistry is the first source devoted to supplying state-of-the-art information on TLC as it applies to the separation, identification, quantification, and isolation of medicinal plant components. Renowned scientists working with laboratories around the world demonstrate the applicability of TLC to a remarkable diversity of fields including plant genetics, drug discovery, nutraceuticals, and toxicology. Elucidates the role of plant materials in the pharmaceutical industry... Part I provides a practical review of techniques, relevant materials, and the particular demands for using TLC in phytochemical applications. The text explains how to determine the biological activity of metabolites and assess the effectiveness of herbal medicines and nutritional supplements. Part II concentrates on TLC methods used to analyze specific plant-based metabolite classes such as carbohydrates, proteins, alkaloids, flavonoids, terpenes, etc. Organized by compound type, each chapter discusses key topics such as sample preparation, plate development, zone detection, densitometry, and biodetection. Demonstrates practical methods that can be applied to a wide range of disciplines... From identification to commercial scale production and quality control, Thin Layer Chromatography in Phytochemistry is an essential bench-top companion and reference on using TLC for the study of plant-based bioactive compounds.

Chemometrics uses advanced mathematical and statistical algorithms to provide maximum chemical information by analyzing chemical data, and obtain knowledge of chemical systems. Chemometrics significantly extends the possibilities of chromatography and with the technological advances of the personal computer and continuous development of open-source software, many laboratories are interested in incorporating chemometrics into their chromatographic methods. This book is an up-to-date reference that presents the most important information about each area of chemometrics used in chromatography, demonstrating its effective use when applied to a chromatographic separation.

The powerful, efficient technique of high performance liquid chromatography (HPLC) is essential to the standardization of plant-based drugs, identification of plant material, and creation of new herbal medicines. Filling the void in this critical area, High Performance Liquid Chromatography in Phytochemical Analysis is the first book to give a complete description of the techniques, materials, and instrumentation of column HPLC and its application to essentially all primary and secondary plant metabolites. Hailing from around the world and with vast expertise in HPLC phytochemical analysis, the contributors present a global, authoritative view of the field. The book looks at the role of HPLC in the analysis of herbal drugs, quality control of plant products in dietary supplements, and chemosystematics. It also covers the phytochemistry, pharmacology, and biological role of plant metabolites as well as various modes and techniques of HPLC analysis. The book then focuses on HPLC separation, identification, and quantification of particular classes of compounds in a variety of sample types, including plants, plant extracts, and plant-derived products. Along with its companion volume Thin Layer Chromatography in Phytochemistry, this comprehensive book presents the most important analytical approaches used in phytochemical analysis. It will help in solving problems connected with practical separations and the analyses of plant extract fractions of active metabolites.

Xenobiotics are chemical compounds foreign to a given biological system. In animals and humans, xenobiotics include drugs, drug metabolites, and environmental pollutants. In the environment, xenobiotics include synthetic pesticides, herbicides, and industrial pollutants. Many techniques are used in xenobiotics residue analysis; the method selected depends on the complexity of the sample, the nature of the matrix/analytes, and the analytical techniques available. This reference will help the analyst develop effective and validated analytical strategies for the analysis of hundreds of different xenobiotics on hundreds of different sample types, quickly, accurately and at acceptable cost.

There is a dramatic rise of novel drug use due to the increased popularity of so-called designer drugs. These synthetic drugs can be illegal in some countries, but legal in others and novel compounds unknown to drug chemistry emerge monthly. This thoughtfully constructed edited reference presents the main chromatographic methodologies and strategies used to discover and analyze novel designer drugs contained in diverse biological materials. The methods are based on molecular characteristics of the drugs belonging to each individual class of compounds, so it will be clear how the current methods are adaptable to future new drugs that appear in the market.

HPLC is the principal separation technique for identification of the pesticides in environmental samples and for quantitative analysis of analytes. At each stage of the HPLC procedure, the chromatographer should possess both the practical and theoretical skills required to perform HPLC experiments correctly and to obtain reliable, repeatable, and reproducible results. Developed to serve as a detailed practical guide, High Performance Liquid Chromatography in Pesticide Residue Analysis is a comprehensive source of information and training on state-of-the-art pesticide residue methods performed with the aid of HPLC. The book presents the pros and cons of HPLC as a flexible and versatile separation and analysis tool with multiple purposes and advantages in investigations of pesticides for food and plant drugs standardization, promotion of health, protection of new herbal medicines, and more.

Used routinely in drug control laboratories, forensic laboratories, and as a research tool, thin layer chromatography (TLC) plays an important role in pharmaceutical drug analyses. It requires less complicated or expensive equipment than other techniques, and has the ability to be performed under field conditions. Filling the need for an up-to-date, complete reference, Thin Layer Chromatography in Drug Analysis covers the most important methods in pharmaceutical applications of TLC, namely, analysis of bulk drug material and pharmaceutical formulations, degradation studies, analysis of biological samples, optimization of the separation of drug classes, and lipophilicity estimation. The book is divided into two parts. Part I is devoted to general topics related to TLC in the context of drug analysis, including the chemical basis of TLC, sample pleparation, the optimization of layers and mobile phases, detection and quantification, analysis of ionic compounds, and separation and analysis of chiral substances. The text addresses the newest advances in TLC instrumentation, two-dimensional TLC, quantification by slit scanning densitometry and image analysis, statistical processing of data, and various detection and identification methods. It also describes the use of TLC for solving a key issue in the drug market-the presence of substandard and counterfeit pharmaceutical products. Part II provides an in-depth overview of a wide range of TLC applications for separation and analysis of particular drug groups. Each chapter contains an introduction about the structures and medicinal actions of the described substances and a literature review of their TLC analysis. A useful resource for chromatographers, pharmacists, analytical chemists, students, and R&D, clinical, and forensic laboratories, this book can be utilized as a manual, reference, and teaching source.

Thin layer chromatography (TLC) is well suited for performing enantioseparations for research as well as larger-scale applications. A fast, inexpensive, and versatile separation technique, there are many practical considerations that contribute to its effectiveness. Thin Layer Chromatography in Chiral Separations and Analysis is the first book to focus solely on the theory, capabilities, and applications of TLC for direct and indirect enantioseparations.

The first part of the book examines the fundamental principles of chirality and TLC. It describes the necessary materials, laboratory equipment, procedures, and strategies for the separation, quantification, isolation, and analysis of chiral compounds. The second part evaluates the real-world enantioseparations and densitometric analyses. Emphasizing pharmaceutical applications, the book discusses chiral separation mechanisms and methods for analyzing the chiral purity of diastereoisomers, amino acids, beta-blockers, and NSAIDS. Topics alsoinclude commercial stationary phases and chiral modifiers of mobile phases.

Thin Layer Chromatography in Chiral Separations and Analysis presents a unified perspective of theory and experimental details underlying the collective developments in the field. The book offers scientists in a variety of disciplines and levels of expertise a complete guide to understanding the current and potential applications of chiral TLC.

Preparative Layer Chromatography explains how this method is used for separating large quantities of mixtures containing a wide variety of important compounds. It offers a broad review of preparative layer chromatography (PLC) applications and adaptable working procedures for microseparations involving organic, inorganic, and organometallic compounds. The book contains theoretical background, chemical principles, and relevance of preparative layer chromatography (PLC) to a wide range of applications, particularly in the study of pharmaceuticals and biochemistry. Written by many of the best known and most knowledgeable specialists in the field, the chapters describe all the necessary techniques, current procedures, and superior strategies for selecting the most suitable eluents and designing application-specific PLC systems based on the data being sought. They provide comprehensive instructions, surrounding issues, and suggestions for optimizing optional working techniques within the framework of PLC. The book also provides a complete coverage of bulk sorbents and precoated chromatographic plates available on the international market. A comprehensive, yet accessible source of information, Preparative Layer Chromatography is a relevant and practical text for experienced as well as novice researchers and practitioners involved in analytical, environmental, geochemical, biological, medicinal, and pharmaceutical analysis.